We have validated CRISPR LifePipe® gRNA design tool by performing a meta-analysis of 10 studies in
human primary cells or cell lines, published between 2016 and 2018.
27 experimentally validated single or paired gRNAs for Cas9sp wt and nickase mutant were extracted
from these publications and studied. All corresponding targets were submitted to CRISPR LifePipe®
gRNA design tool, and the ranking of gRNAs was analyzed.
Firstly, we found that all published gRNAs were found by our application, indicating its high
level of accuracy. Moreover, we observed that most of them ranked in the 3 best gRNAs returned
by our application. All of these results prove the quality of the CRISPR LifePipe® gRNA design
tool and its ability to help scientists to choose the best gRNAs for their experiments.