Our Lab


In 2020, we started a collaboration with the Institute of Biology François Jacob, CEA. Thanks to this new opportunity, the IDMIT department welcomed us in their premises.

Specialized in Infectious Disease, IDMIT department excels in the development of new therapeutic strategies and new generation vaccines in the preclinical non-human primate model.

We therefore benefit from top research laboratories in BSL1, BSL2 and BSL3 containment conditions that are certified ISO 9001-2015.




Our Offer


Our core facility supports the sequencing processing from study design to data analysis with innovative high-end solutions.

Our experience is enhanced by good sample management, DNA extraction and storage.

Multiple bioinformatic services for data analysis are equally available.

Applications



Whole Genome

Sequence whole genome for a complete insight of genomic content and variations.

Targeted Sequencing

Detect known and novel variants within a region of interest.

Metagenomic

Characterize mixed populations of microorganisms.

Virus Detection

Detection of genomic mutations for variant diagnosis.

Transcriptomic

Explore transcriptome to determine an organism expression profile.

Epigenomic

Investigate clues of gene regulation with epigenetics and analyse DNA methylation profiles.

Sequencing Technologies

Our platform offers sequencing analysis from samples for different applications. We can provide a precise microbial composition in environmental or biological samples and analyze transcriptomic or epigenetic profiles. The processing of acquired data can put forward modifications or gene expression.
According to project design, we can exploit different approaches.

Nanopore


Nanopore technology based on electrical current changes interpretation when nucleic acids pass through a nanopore protein, allows us to sequence any DNA/RNA fragment to ultra-long reads.

It is possible to sequence complete bacterial and viral genomes with long read sequencing. And thus identify and characterize de novo viral variants and bacterial strains.

Long reads also allow to identify precise mutations in eucaryotic samples (insertions, deletions, base modifications) to identify novel cancer biomarkers.

Devices: MinION, GridION



Illumina

The Illumina next generation sequencing (NGS) technology is based on amplification of DNA and sequencing by synthesis. It allows a wide range of sequencing analysis with high accuracy.

The sequencing of the hyper variable regions (16S, 18S) is a classical method to characterize a microbiota and determine bacterial abundance.

Bacterial specific 16S rDNA sequencing allows an efficient Genus discrimination.

Devices: MiSeq



Chromium 10X Genomics


The Chromium 10X technology allows single cell RNA-seq. It is based on partitioning individual cells of a complex sample into a lipid droplet using a microfluidic system. Barcoding of each cell and transcript with a unique barcode allows single cell transcriptomic analysis.

We use 10X Cell Ranger together with an in-house pipeline to eliminate biased cells (dying cells, doublets, empty droplets) and characterize a complex sample. Transcriptomics data are analysed to determine activation profiles on identified clusters of cells and even explore differences in expression.

Devices: Chromium and NextSeq2000 (on demand)



Contact Us

Ask about our offer, find answers to your questions and get in touch.